Method: Sixty-four denture wearers patients (six men and 58 women, mean age 57,1y.r.) were included in the study and submitted to a swab collection for culture and isolation of Candida sp, 47 of which presented denture stomatitis (two men and 45 women). The samples were obtained by swabbing the palate, buccal mucosa and the acrylic denture base. Forty samples (32 from denture stomatitis patients) were positive for Candida sp, that once isolated were submitted to progressive concentrations of antiseptics (CPC and TRI) and anti-fungals (nystatin[N] and ketoconazol[K]). Data were recorded as MIC (minimum inhibitory concentration) and MFC (minimum fungicidal concentration), according to the minimum concentration that prevented visible growing of colony forming units and the minimum concentration that prevented growing in sub-cultured sabouraud dextrose agar plates, respectively.
Result: The results were MIC and MFC of 0,07g/L for CCP, MIC 2,5g/L and MFC 10g/L for TRI, MIC 16ug/ml and MFC 64ug/ml for K, and MIC and MFC of 25UI/ml for N. The antiseptics solutions that showed fungicidal properties present concentrations encountered in the commercially available products.
Conclusion: Based on these results we concluded that the antiseptics assayed were effective against Candida sp and that CPC was more effective than TRI in this in vitro study.