IADR Abstract Archives

Photodynamic efficacy of curcumin  and blue LED on 

Objective: This in vitro study investigated the effect of photodynamic therapy (PDT) with curcumin dye (C) and a light-emmiting-diode (LED) (L) in the blue wavelength  on the viability of Streptococcus mutans (ATCC 25175) cells in a planktonic culture.

Method: Planktonic suspensions were analyzed in four different groups: C-L- (no die, no light; control group), C+L- (three concentrations of curcumin:0.75, 1.5 and 3.0 mg /mL), C-L+ ( three light dosimetries:15, 30, and 45J/cm2) and the PDT group  (C+L+). Aliquots of each group were transferred to Petri dishes for counting of colony forming units (CFU/mL). The data were analyzed by Kruskal-Wallis and Student Newman Keels test (p<0.05).

Result: In comparison to control group, the isolated effect of the light (30J/cm2, p = 0.01 and 45J /cm2, p = 0.01) showed a reduction of 5% to those wavelengths. The effect of the dye showed no influence on the bacterial reduction. In relation to PDT group the associations of (C)1.5 mg/mL and (L) 30J/cm2 (p = 0, 04), (C)1.5mg / mL and (L) 45J/cm2 (p = 0.03) and (C)3mg/mL and (L) 45J/cm2 (p = 0.03) showed a bacterial reduction of 39.9%, 29% and 38.1%, respectively. 

Conclusion: The results demonstrated that, in the parameters studied, PDT has demonstrated effectiveness in the inactivation of S. mutans.

Division: IADR/LAR General Session
Meeting: 2012 IADR/LAR General Session (Iguaçu Falls, Brazil)
Location: Iguaçu Falls, Brazil
Year: 2012
Final Presentation ID: 1496
Abstract Category|Abstract Category(s): Cariology Research - Microbiological Studies / Biofilm
Authors
  • Coradi Tonon, Caroline  ( Araraquara School of Dentistry UNESP Univ Estadual Paulista, Araraquara, N/A, Brazil )
  • Bagnato, Vanderlei  ( USP University of Sao Paulo Physics Institute of São Carlos, São Carlos, N/A, Brazil )
  • Spolidorio, Denise Palomari  ( UNESP - Dental School of Araraquara, Araraquara - SP, N/A, Brazil )
  • Giusti, Juçaíra  ( USP University of Sao Paulo Physics Institute of Sao Carlos - SP, Sao Carlos, N/A, Brazil )
  • Paschoal, Marco Aurélio  ( Universidade Est. Paulista Julio Mesquita, Araraquara-SP, N/A, Brazil )
  • Santos Pinto, Lourdes  ( Faculdade de Odontologia de Araraquara, UNESP Univ Estadual Paulista, Campus Araraquara, Araraquara, N/A, Brazil )
  • SESSION INFORMATION
    Poster Session
    Cariology: Cariogenic Biofilm Inhibitors
    06/21/2012