IADR Abstract Archives

Effect of Vwc2 Protein on Calvarial Bone Formation

Objective: We have recently reported that a novel secretory protein, von Willebrand factor C domain containing 2 like (Vwc2l), promotes osteoblast mineralization in vitro. In the present study, Vwc2, a highly homologous protein to Vwc2l was characterized.The objective of our study is to investigate the effect of Vwc2 on bone formation.

Method: The expression of Vwc2 in MC3T3-E1 (MC) osteoblastic cell line was investigated by real time PCR at RNA level and by Western Blotting (WB) analysis at protein level. The presence of Vwc2 protein in bone was also examined by immunohistochemistry (IHC) and WB analysis. To characterize the function of Vwc2 protein,recombinant Vwc2 protein (rVwc2) was added into mouse calvaria ex vivo cultures and the extent of newly synthesized bone formation was analysed.

Result: Vwc2 transcript is detected in MC cells with the highest expression observed at day 7 during biomineralization. The expression pattern of Vwc2 protein in the cultured media from MC cells was similar to that of Vwc2 transcript. The localization of Vwc2 protein was observed by IHC in mouse developing maxilla. The effect of rVwc2 on mouse calvaria bone formation was examined and the results showed increased bone formation. 

Conclusion: Our data demonstrated that Vwc2 is expressed in osteoblasts/bone and has the ability to increase bone formation. The data obtained from this study may provide insights to the biological functions of this novel protein and help to develop a new molecular design for bone loss therapies.

IADR/LAR General Session
2012 IADR/LAR General Session (Iguaçu Falls, Brazil)
Iguaçu Falls, Brazil
2012
2941
Craniofacial Biology
  • Almehmadi, Ahmed  ( Boston University, Boston, MA, USA )
  • Ohyama, Yoshio  ( Boston University, Boston, MA, USA )
  • Jaha, Haytham  ( Boston University, Boston, MA, USA )
  • Venkitapathi, Sundharamani  ( Boston University, Boston, MA, USA )
  • Aljamaan, Reem  ( Boston University, Boston, MA, USA )
  • Kaku, Masaru  ( Niigata University Graduate School of Medical and Dental Sciences, Niigata, N/A, Japan )
  • Mochida, Yoshiyuki  ( Boston University, Boston, MA, USA )
    • Poster Session
    2012 Sarnat Competition
    06/22/2012