IADR Abstract Archives
The effect of lidocaine and benzocaine on keratinocytes’ cytokines release
Method: Human keratinocytes (HaCaT) were grown in DMEM medium supplemented with 10% heat-inactivated FBS and 50 µg/mL gentamicin. The cells were exposed to 10 µM lidocaine or 10 µM benzocaine for 6 hours. Cytokines quantification was performed by ELISA and cell viability was estimated by using XTT assay.
Result: Table 1 shows the results (mean±SEM). None statistically significant differences (Kruskal-Wallis, p>0.05) were observed among groups regarding IL-1alpha, IL-6 and IL-8 levels. Both benzocaine and lidocaine attenuate (p<0.05) the cell production of TNF-alpha in relation to the control group. Benzocaine induced less (p<0.05) cell growth than the other groups.
Control |
Benzocaine |
Lidocaine |
|
IL-1alpha (ng/mL) |
48.6 (±6.5) |
28.3 (±3.9) |
36.9 (±6.1) |
IL-6 (ng/mL) |
192.8 (±24.7) |
117.7 (±20) |
128.6 (±11.1) |
IL-8 (ng/mL) |
19.6 (±3.5) |
13.6 (±1) |
15.1 (±4.3) |
TNF-alpha (ng/mL) |
43.4 (±10.4) |
18 (±1.5) |
11.4 (±2.3) |
Cell growth (%) |
99.0 (±3.1) |
84.9 (±4) |
105 (±3) |
Conclusion: Both local anesthetics reduced the production of TNF-alpha by the keratinocytes, but benzocaine showed more cell toxicity than lidocaine.