Method: We studied the inhibitory effect of Chrysanthemum boreale essential oil on the biofilm formation of S. mutans and on the adhesion of S. mutans to hydroxyapatite beads. The chemical constituents of the essential oil were analysized by GC/MS.
Result: The essential oil of Chrysanthemum boreale inhibited the biofilm formation of S. mutans on the surface of resin teeth and also inhibited the adhesion of S. mutans to hydroxyapatite beads at the concentration of 0.05, 0.1, 0.25, 0.5, 0.75, and 1 mg/ml compared to the control group. The major constituents of essential oil of Chrysanthemum boreale are Camphor (20.89%), β-caryophyllene (5.71%), α-thujone (5.45%), piperitone (5.27%), epi-sesquiphellandrene (5.16%), α-pinene(4.97%), 1,8-cineole (4.52%), β-pinene (4.45%), and camphene (4.19%).
Conclusion: These results suggest that essential oil of Chrysanthemum boreale may inhibit the biofilm formation of S. mutans. Camphor (20.89%), β-caryophyllene (5.71%), α-thujone (5.45%), piperitone (5.27%), epi-sesquiphellandrene (5.16%), α-pinene(4.97%), 1,8-cineole (4.52%), β-pinene (4.45%), and camphene (4.19%), the major constituents of Chrysanthemum boreale essential oil may be responsible for such biomolecular activities.