to determine the anti-bacterial activity of the essential oil of Satureja brevicalix Epling “muña” (50% and 100%) compared 0.2% clorhexidine against Fusobacterium nucleatum ATCC 25586 and Porphyromonas gingivalis ATCC 33277 strains.
Method:
The sample was composed of 18 petri plates for Fusobacterium nucleatum ATCC 25586 and 19 plates for Porphyromonas gingivalis ATCC 33277. These bacterial strains were cultured in Columbia agar containing 5% of defibrinated sheep blood, with hemin (0,5%) and vitamin K (0,1%), and incubated for 48 hours at 37°C. The antibacterial tests were carried out using diffusion discs absorbed with the essential oil of Satureja brevicalyx Epling “muña” (50% and 100%) and clorhexidine at 0,2%.
Result:
The essential oil of Satureja brevicalyx Epling “muña” (50% and 100%) had antibacterial activity in both pathogens and it was statistically significant in the concentration of 100% in comparison with the one of 50% (p<0.001), the comparation of Satureja brevicalyx Epling “muña” at 100% with clorhexidina at 0,2% was not statistically significant difference (p>0.001) in both bacterial strains; thus the plant in study presents similar antibacterial activity if we compare with group control.
Conclusion:
the essential oil of Satureja brevicalyx “Muña” (50% and 100%) showed antibacterial activity against Porphyromonas gingivalis ATCC 33277 and Fusobacterium nucleatum ATCC 25586 strains.