The aim of this study was to investigate whether FokI, BsmI polymorphisms of the VDR genes are associated with the presence of generalized aggressive periodontitis (GAP).
Method:
The study population consisted of 70 GAP patients and 128 control including 32 periodontally healthy subjects (aged 21 to 30 years) and 96 children, recruited from the Departments of Periodontics and Pedodontics, Chonnam National University Dental Hospital. Genomic DNA was extracted from buccal swab and FokI, BsmI polymorphisms were determined by PCR-RFLP analysis. The frequency of genotype and alleles, and haplotype was analysed by chi-square test.
Result:
In the GAP group, the frequencies for FokI genotypes of C/C, C/T, and T/T were 34.3%, 50.0%, and 15.7% respectively and the frequency of T allele was 41%. There was no significant difference in the distribution of genotypes nor allele between two groups.
As for BsmI polymorphisms, the frequencies for genotypes of G/G, G/A, and A/A in the GAP group were 84.5%, 15.5%, and 0.0% respectively and the frequencies of A allele was 8%. There was no significant difference in the distribution of genotypes nor allele between two groups.
The haplotype analysis of the two combined VDR gene polymorphisms revealed there was no significant difference in the distribution of haplotypes between the groups.
In female, the frequency of A allele in BsmI polymorphisms of GAP was significantly lower than that of control (p<0.05). The analysis of haplotypes of the two combined VDR gene polymorphisms revealed the female TG homozygous subjects had a significantly higher prevalence of GAP (OR=7.3, 95% CI: 0.82 - 65.12, p<0.05).
Conclusion:
According to these findings, the BsmI genotypes and FokI/BsmI haplotypes of VDR gene polymorphisms might be associated with GAP Korean female people.