Methods: Samples of 100 mg fresh mixed or set materials were prepared and then eluted with 1mL cell culture medium for the working time period (fresh) or for 6 hours (set), respectively. L929 cells were seeded into 96-well plates at 5x103 cells/well and incubated with 200µl eluate from each eluate group. Cells cultured with culture medium only served as controls (100%). After 24 hours’ incubation the cytotoxicity was evaluated by MTS assay. Cell viability was calculated as the percentage of the control group. ANOVA test was conducted to investigate if there were significant differences in cell viability between materials and control, and Student’s t-tests were conducted to identify which material was significantly different from control with Dunnet’s correction. Cell viability among material groups was also compared using ANOVA test and Student’s t-tests with Bonferroni correction.
Results: In fresh, average±S.D.(%) of cell viability were 40.2±14.0, 43.7±16.0, 72.9±12.7, and 66.0±13.6 for Dycal, Life, MTA, and SB-C&B, respectively. Dycal (p=0.0124) showed statistical significance when compared with control. In set, average±S.D.(%) of cell viability were 48.7±14.8, 37.2±10.6, 46.7±15.2, and 100±21.9 for Dycal, Life, MTA, and SB-C&B, respectively. There was no statistical difference in cell viability among material groups in fresh and set conditions.
Conclusions: Life, MTA, and SB-C&B were less cytotoxic than Dycal, if the material was fresh. There were no significant differences in cytoxicity among Dycal, Life, MTA, and SB-C&B, if the material was set. Supported by NIH KL2RR024983(TK)/UL1RR024982.