salivary and GCF Il-1beta levels before and after periodontal therapy

Introduction:Periodontal disease is a chronic inflammatory disease characterized by connective tissue breakdown and alveolar bone loss. Radiographic assessments and clinical measurements such as gingival index, bleeding on probing, probing depth, clinical attachment level are conventional diagnostic methods to determine periodontal disease presence and severity. As the limitations of these traditional methods became clear, now techniques have been proposed as diagnostic tests for periodontal disease. In this study we wanted to assess whether there is any correlation between GCF and saliva proinflammatory cytokine levels during periodontal disease. Material and Methods:The study group consisted of 16 moderate to severe chronic periodontitis patients and 17 healthy subjects. A medical history was reported to rule out any systemic desorder that could potentially affect periodontal status. The enrollment criteria were a minimum of 20 natural teeth, excluding third molars. Clinical data included probing depth, beeding on probing were collected at six sites per tooth. Patients with chronic periodontal disease were of required to have at least six sites with equal or more than 5mm probing depth. Subjects were asked to expectorate 10mL of unstimulated whole saliva into a 50-ml sterile plastic sentrifuge tube. Following isolation from saliva and removal of supra gingival plaque, filter paper strips were introduced mesiobuccal gingival sulci of all teeth. All teeth except third molars were sampled for GCF. The filter strips were than placed in ependorf tubes. Results:At baseline total GCF levels of IL-1beta was significantly higher at perºodontitis group than control group. After periodontal treatment GCF IL-1beta levels decreased. IL-1beta saliva levels did not show any significant difference either between control and periodontitis patients or before and after periodontal therapy. Conclusion: IL-1beta GCF levels might be a diagnostic marker for periodontal disease,however GCF levels did not reflect to saliva levels.