Effect of centrifugal force on biological properties of platelet-rich fibrin
Platelet-rich fibrin (PRF) is a second-generation blood plasma-and-platelet derivative. It is prepared by centrifugation of coagulating blood. OBJECTIVE: Analyze content and distribution of platelets in PRF after spinning at different centrifugation speeds. METHOD: Coagulating human venous blood was spun at relative centrifugal force 208, 470, 834 and 1303 g for 20 minutes. Slices of coagulum were stained with a platelet-specific antibody (CD41, BD Pharmingen). Density and position of platelets along the longitudinal axis were determined by fluorescence microsopy. RESULTS: A low centrifugation force yielded a PRF coagulum contained more platelets that were widely distributed. A high centrifugal force yielded a PRF coagulum with platelets concentrated in its peripheral end. CONCLUSION: Centrifugal force is an important determinant of density and distribution of platelets in PRF coagulum. PRF is used to improve healing and regeneration of damaged tissues. PRF with a higher content of evenly distributed platelets may be clinically more efficient. This pilot study was supported by the Research Pilot Project Award 03 from the UOP Arthur A. Dugoni School of Dentistry, San Francisco, CA.
Division: IADR/AADR/CADR General Session
Meeting:2011 IADR/AADR/CADR General Session (San Diego, California) Location: San Diego, California
Year: 2011 Final Presentation ID:3535 Abstract Category|Abstract Category(s):Oral & Maxillofacial Surgery
Authors
Tolar, Mirek
( University of the Pacific, San Francisco, CA, USA
)
Baker, Eric
( Dugoni School of Dentistry, University of the Pacific, San Francisco, CA, USA
)
Betita, Timothy
( Dugoni School of Dentistry, University of the Pacific, San Francisco, CA, USA
)
Hansen, Preston
( Dugoni School of Dentistry, University of the Pacific, San Francisco, CA, USA
)
Keyes, Lance
( Dugoni School of Dentistry, University of the Pacific, San Francisco, CA, USA
)
Nattestad, Anders
( Dugoni School of Dentistry, University of the Pacific, San Francisco, CA, USA
)
SESSION INFORMATION
Poster Session
Oral & Maxillofacial Surgery IV
03/19/2011