Comparative Antifungal-activity of Light-activated Disinfection with Octenisept and Contemporary Irrigants

Methods: Seventy extracted single-rooted human teeth were decoronated and root canals were instrumented using ProTaper files (Dentsply-Maillefer,Ballaigues,Switzerland) to size F3. The smear layer was removed before sterilization (121°C,40 min). After incubation period of 14 days with Candida albicans(ATCC 90028), the samples were divided into 5 experimental groups (n=10); LAD with toluidine blue O (TBO) (the irradiation time was 30 seconds as the manufacturer's recommendations); Octenidine-hydrochloride (OCT), 5.25% sodium hypochlorite (5.25% NaOCl), 2.5% sodium hypochlorite (2.5% NaOCl), 2% chlorhexidine (CHX) (five mL of each test solution was applied for 3 minutes). There were two control groups (n=10); (positive; infected samples and negative; sterile samples). After the treatments, dentin chips were collected from inner root canal walls with gates-glidden burs (#3, 4 and 5) into vials containing 2 mL of phosphate buffered saline, vortexed for 30 s, serially diluted to 10^-4and seeded on tryptic soy agar plates and incubated (37° C, 48h). The number of colony-forming units was calculated and statistical analysis was performed (Kruskal Wallis and Mann Whitney-U tests).

Results: Octenidine-hydrochloride, 5.25% NaOCl, 2.5% NaOCl and 2% CHX groups totally eradicated all Candida cells (CFU=0). LAD group reduced C. albicans 10.16% relative to the positive control which was not statistically significant (P>0.05).

Conclusions: New wound-antiseptic Octenidine-hydrochloride demonstrated the total elimination of Candida albicans cells and may be a promising alternative to sodium hypochlorite and chlorhexidine solutions in the future. LAD treatment had no significant antifungal effect on this yeast. Further modifications in the LAD protocol may be required to increase its efficacy.