IADR Abstract Archives
Evaluation of Cytotoxicity of Pharmaceutical Grade Alginates on HeLa Cells
Objective: To evaluate the cytotoxicity of various pharmaceutical grade sodium alginates with different guluronate/mannuronate residues on the viability of the HeLA cells.
Methods: Listed in the following table, six pharmaceutical grade sodium alginates (FMC Biopolymers) were used in this study. Alginate beads were prepared by extruding 2wt% sodium alginate solutions through a syringe fitted with 25G needle directly into an aqueous solution of 100mM CaCl2. Alginate beads weighing ~30, 60 and 120mg were placed into each well of a 24-well culture plate containing 30 to 40% confluent monolayers of the HeLA cells. Following 48 hours of incubation, cell viability was evaluated by a standard colorimetric MTT assay.
Results: Viability data (mean±SD, n=3) for treated cells compared to control (cells without beads) is listed in the following table. Compared to the control group, cell viability was not altered for the cells treated at lower alginate doses. In contrast, groups 5 and 6, cell viability was significantly reduced by 20-40% when exposed to higher doses of alginates, among all groups.
GROUP | Sodium Alginate | G/M ratio | Viscosity | Cell viability % @ 30mg
| Cell viability % @ 60mg
| Cell viability % @ 120mg
|
1 | Protanal LF240D | 30-35/65-70 | 70-150 | 97.73±0.045 | 96.5±0.018 | 61.03±0.075 |
2 | Protanal LF10/60LS | 35-45/55-65 | 20-70 | 98.06±0.048 | 86.49±0.021 | 73.15±0.035 |
3 | Protanal LF120M | 35-45/55-65 | 70-150 | 88.23±0.03 | 89.88±0.036 | 78.13±0.06 |
4 | Protanal LF10/60 | 65-75/25-35 | 20-70 | 109.96±0.046 | 97.66±0.03 | 74.44±0.02 |
5 | Protanal LF200S | 65-75/25-35 | 200-400 | 119.18±0.021 | 112.72±0.028 | 91.7±0.045 |
6 | Protanal LFR5/60 | 65-75/25-35 | 300-700 | 102.11±0.032 | 91.61±0.035 | 95.83±0.067 |
Conclusions: The viability of HeLA cells was found to be dependent on the amount of the alginate used. Maximum viability was obtained with 30 and 60mg treatments. Over the entire weight range, alginates with high G content displayed better viability.