Objective: Infections of the oral cavity (e.g. oral candidiasis and gingivitis) are important comorbidities of dry mouth, which are caused by fungi and bacteria. In this study, the antifungal/antibacterial activities of Oasis™ (Tri-Hydra™ technology) and Biotene™ (enzyme mix) mouthwash solutions were compared.
Method: Tested isolates were: Candida spp. [C. albicans (CA), C. krusei (CK), C. tropicalis (CT), and C. glabrata (CG)], [Streptococcus sanguis (SS), S. oralis (SO), S. mitis (SM), S. salivarius (SSA), S. gordonii (SG), Staphylococcus aureus (SA), Actinobacillus actinomycetemcomitans (AA), and Fusobacterium nucleatum (FN). Activity of Oasis™ and Biotene™ was evaluated by determining their minimum inhibitory concentrations (MICs) against each isolate. MIC was calculated according to CLSI M27-A3, M07-A8, M11-A7 reference methods for susceptibility testing of yeasts, aerobic bacteria, and anaerobic bacteria, respectively. MIC endpoints for yeast and bacteria were defined as concentrations (%) that inhibit 50% and 100% growth, respectively. Effect of Oasis™ and Biotene™ on ultrastructure of SS and SO was evaluated using electron microscopy (EM).
Results: MIC of Oasis™ against CA, CK, CT and CG was 0.05%, 0.0125%, 0.0125% and 0.025%, respectively, while that of Biotene™ against these organisms was 6.25%, 3%, 25%, and 6.25%, respectively. MIC of Oasis™ against SO, SM, SSA, SG, SA, AA, FN, and SS was 6.25%, 0.8%, 0.1%, 25%, 25%, 0.2%, 0.4%, and 50%, respectively. In contrast, Biotene™ MICs were 50%, 50%, 50%, 50%, 50%, 6.25%, 6.25%, and 50%, respectively. EM analysis showed that Oasis™ treatment of SS and SO affected the morphology of these bacteria, resulting in deformed “cauliflower-like” morphology and cell disruption. In contrast, treatment with Biotene™ (at the same concentration) did not affect morphology of these organisms.
Conclusions: Oasis™ possesses potent antifungal and antibacterial activity and caused cellular disruption. This activity was superior to Biotene™, which showed antimicrobial activity at high concentrations only.
AI-U01-68636, Oasis HealthCare