IADR Abstract Archives

Efficacy of Endodontic Irrigant Solutions in Killing Polymicrobial Biofilm

Objectives: To compare the in vitro efficacy of endodontic irrigating solutions in the killing of four-species biofilms. Methods: Fifteen extracted human teeth were sterilized (10% Formalin) and stored (ultrapure water). Coronal and root portions of teeth were separated and canals were prepared in sequence: 50\.04, 40\.04, 35\.06, 30\.06 (25mm Vortex rotary files, Dentsply Tulsa). Prepared roots were cut vertically (sagittal sections) and sections were divided into three groups (n=10/group) and sterilized (100%isopropanol/15min; UVPCL-1000 crosslinker/10min). Polymicrobial biofilms (E. faecalis ATCC4083, F. nucleatum ATCC25586, P. gingivalis ATCCBAA308 and P. nigrescens ATCC33563) were grown anaerobically on the sections (3 days, 37°C). Irrigant solutions tested were 6.15% Sodium hypochlorite (BLCH), 2.00% Chlorhexidine gluconate (CHXP) and 17.00% EDTA (EDTA). Root sections (n=8/group) were immersed in one of the irrigants and two root sections/group were immersed in water (control; CTRL). Sections were stained (Backlight LIVE/DEAD, Invitrogen) and imaged (Leica TCS SP2 MP). Biofilm structural parameters calculated using ISA3-D software were: average diffusion distances (AD), fractal dimension (FD) and mean biofilm thickness (MT). Parameters were compared using General-Linear-Models and post-hoc Student-Newman-Keuls tests (SNK;α=0.05) to determine differences in biofilm structures for Syto-9/Live and Propidium Iodide/Dead stains at three locations on each section. Results: Statistically significant differences existed among the irrigants tested (p<0.01), and between stains (p<0.01). No statistically significant differences existed among the locations on the root sections (p>0.05).

LIVE

DEAD

Group

ADD(µm)

MT(µm)

ADD(µm)

MT(µm)

BLCH

0.73±0.00^C

2.84±0.04^A

152.07±108.04^A

0.75±0.01^C

2.76±0.09^A

134.03±83.13^A,B

CHXP

0.94±0.10^A

2.44±0.13^C

76.60±34.98^B

0.97±0.12^A

2.49±0.19^B

91.53±41.64^B

EDTA

0.87±0.07^B

2.44±0.13^C

123.98±60.64^A

0.85±0.11^B

2.45±0.17^B

138.94±70.24^A,B

CTRL

0.89±0.14^A,B

2.54±0.12^B

136.22±91.77^A

0.85±0.10^B

2.54±0.16^B

163.91±90.94^A

* Superscript letters denote SNK rankings by column (p<0.01)

Conclusions: Sodium hypochlorite solution was most effective at killing the polymicrobial biofilms tested. A protocol was established for the in vitro study of endodontic treatment modalities in prepared root canals in human teeth. Funding: Dentsply Tulsa Dental Specialties.

Division: IADR/AADR/CADR General Session
Meeting: 2011 IADR/AADR/CADR General Session (San Diego, California)
Location: San Diego, California
Year: 2011
Final Presentation ID: 41
Abstract Category|Abstract Category(s): Dental Materials 5: Biocompatibility and Biologic Effects

Authors

Khajotia, Sharukh S. ( University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA )

Merritt, Justin ( University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA )

Goldbeck, Andrew ( University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA )

Smart, Kristin H. ( University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA )

Dmytryk, John J. ( University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA )

SESSION INFORMATION

Oral Session
Biologic Effects of Dental Materials
03/16/2011