IADR Abstract Archives

Osterix Enhances BMSC-Association Osseointegration of Implants

Objectives: The purpose of this study is to identify the source of cells involved in the processes of osteogenesis after implantation, and to elucidate the roles of Osx in osteogenic differentiation at the interface between host bone and titanium implants.

Methods: BSP9.0Luc/ACTB-EGFP mice contain a luciferase gene driven by BSP promoter and an enhanced green fluorescent protein (EGFP) driven by a beta-actin promoter. Bone marrow stromal cells (BMSCs) isolated from these mice were transplanted into 4-week-old nude mice through intracardiac injection. Five weeks after the transplantation, implants were inserted into the femurs of these nude mice. The femurs were dissected at d7 (day 7) and d21 after implantation. BSP/TVA mice, in which an avian retroviral receptor gene is hooked to a mouse BSP promoter, were divided into two groups. Implants were inserted into the femurs after administration of RCAS-OSX virus or empty virus. The femurs were dissected for microCT, RT-PCR, H&E staining and IHC staining at d7 and d14 after implantation.

Results: In BMSCs transplanted nude mice, the ratio of luciferase positive cells to GFP positive cells detected at d7 was higher than that at d21. In BSP/TVA mice, 14 days after implantation, microCT analysis showed an increased bone density at the bone-to-implant interface in RCAS/Osx group compared with the control group. As shown by RT-PCR analysis, levels of Osx, BSP and osteocalcin (OC) were higher in RCAS/Osx group than in the control group at d7 and d14. Histological analysis demonstrated that cartilage-bone cells were observed at the bone-to-implant interfaces in RCAS/Osx group at day 7. IHC revealed active expression of BSP and OC in the newly formed bone in RCAS/Osx group.

Conclusion: Exogenous BMSCs underwent osteogenic differentiation and participated in the osseointegration. Overexpression of Osx could accelerate bone regeneration process at bone-to-implant interfaces.


AADR/CADR Annual Meeting
2010 AADR/CADR Annual Meeting (Washington, D.C.)
Washington, D.C.
2010
41
Implantology Research
  • Beiyum, Xu  ( Tufts University, Boston, MA, USA )
  • Zhang, Jin  ( Tufts University, Boston, MA, USA )
  • Brewer, Erika  ( Tufts University, Boston, MA, USA )
  • Tu, Qisheng  ( Tufts University, Boston, MA, USA )
  • Kim, Min  ( Tufts University, Boston, MA, USA )
  • Wieland, Marco  ( Institute Straumann AG, Basel, N/A, Switzerland )
  • Chen, Jake Jinkun  ( Tufts University, Boston, MA, USA )
    • Oral Session
    Bone-Implant Interface
    03/03/2010