Methods: The test procedure utilized for this investigation was similar to the one identified as Procedure 40 in the U.S. Food and Drug Administration's 2003 monograph on anticaries drug products for over-the-counter human use1, with the following exceptions: 1. The lesion was formed using a solution of 0.1M lactic acid and 0.2% Carbopol 907 and was 50% saturated with HAP at a pH of 5.0. 2. Shorter treatment times and timed dilutions of artificial saliva were utilized to better mimic clinical conditions. Twenty four specimens were evaluated per treatment group. Twelve specimens were evaluated for fluoride immediately following treatment and twelve specimens were evaluated for fluoride 12 hours post treatment. This study evaluated seven parallel groups of enamel specimens
Results: This study showed that the fluoride containing mouthrinses promoted significant fluoride uptake into the incipient lesions as compared to the water control, 12 hours after dosing (p <0.001) as well as immediately following treatment (p <0.001).
Conclusions: Listerine® Total Care, Listerine® Total Care (UK), Listerine® Teeth & Gum Defence, Listerine® Smart Rinse ™ , an experimental hydrogen peroxide and fluoride containing mouthrinse and Experimental CPC and fluoride containing mouthrinse were effective in increasing the amount of fluoride in enamel immediately following one treatment ( 30 or 60 second) as well as 12 hours post treatment when compared to a fluoride-free negative control.
References: 1. Test Method #40: Determination of Fluoride Uptake by Enamel. http://www.fda.gov/OHRMS/DOCKETS/dockets/80n0042/80n-0042-bkg0001-toc.htm