Methods: Two commercially available collagen membranes were tested; Ossix Plus (OP, OraPharma, GLYMATRIX cross-linked porcine type I collagen), OsseoGuard (OG, Biomet 3i, noncross-linked bovine type I collagen. MC3T3-E1 mouse preosteoblasts were cultured with OP or OG collagen membrane. The cell culture without membranes was used for the control. After 2-day culture, the cells were collected, and key osteogenic and extracellular matrix gene expressions (BMP-2, type II collagen, osteopontin, and Runx2) were analyzed by quantitative real-time PCR.
Results: With OP membrane, gene expression levels of BMP-2, type II collagen, osteopontin, and Runx2 slightly decreased compared to the control, but the differences were not significant. With OG membrane, although gene expression levels of BMP-2 slightly increased and of type II collagen and Runx2 slightly decreased compared to the control, the differences were not significant. However, only osteopontin expression level in osteoblasts with OG membrane significantly decreased compared to the control.
Conclusion: In 2-day cultured preosteoblasts, collagen membranes had little effect on gene expression levels of bone-related matrix, except osteopontin inhibited with OG membrane