Methods: Following one-week of brushing with a fluoride-based toothpaste, each of ten healthy subjects provided 10 mL of unstimulated morning saliva prior to brushing, eating, and drinking. Saliva (2 mL) was transferred to a centrifuge tube and incubated for 24 h at 37 oC. Each sample was prepared in triplicate. The samples were then extracted with hexanes and analyzed for indole by GC-MS. Two commercially-available mouthrinses were evaluated for their effect on indole concentration, one containing AmF/SnF2 and zinc lactate and the other containing CPC and alcohol (15%). Water was used as a control. Following one week of brushing with a fluoride-based toothpaste, stimulated morning saliva was collected and pooled together from five healthy subjects. The saliva was provided prior to brushing, eating, and drinking. Saliva (2 mL) and tryptophan (0.3 mL, 1.09% in water) was transferred to a centrifuge tube, mixed by vortex, and incubated for 1 h at 37 oC. The treatment product (40 μL) was added to the saliva mixture, mixed by vortex, and incubated for 23 h at 37 oC. Each sample was prepared in triplicate. The samples were then extracted with hexanes and analyzed for indole by GC-MS.
Results: The mean indole content in unstimulated morning saliva is 14.7 ± 10.5 ppm. The results of the in-vitro test showed that the amounts of indole in saliva following the treatment are: 64.5 ± 2.2 ppm with water, 62.3 ± 1.1 ppm with mouthwash containing CPC and alcohol (15%), and 34.0 ± 2.4 ppm with mouthwash containing AmF/SnF2 and zinc lactate.
Conclusions: Mouthwash containing AmF/SnF2 and zinc lactate significantly (p<0.05) reduced the indole amount in saliva compared to mouthwash containing CPC and alcohol (15%) and the control.