Objectives: Degradable scaffolds are widely used in tissue engineering. During the degradation period chemical and morphological alterations -which could affect cell proliferation-, take place. The aim of this study was to investigate alterations in surface morphology of chitosan (CHS-CC) and chitosan-gelatin (CHS-G-CC) composite ceramics, during incubation in organic medium (DMEM), with or without cells. Methods: Two types of composite ceramic scaffolds were fabricated with the method of leaching water non-soluble particulates, on the surface of modified ceramic disks (MCD), with a mixture of bioactive glass – ceramic (1:1 wt): CHS-CC and CHS-G-CC. MCD served as controls. Four specimens were incubated in DMEM with and four without human PDLF cells, for 10 days. SEM microphotographs were used to observe surface morphology and pores structure and analysis of the elemental composition was performed (EDS). The MTS method was used to evaluate cell proliferation. Results: Constructed scaffolds appeared to be macro porous with high interconnectivity and pores size 70-200μm. SEM microphotographs revealed that cells were capable of adhering to all specimens, after cell seeding. In case of CH-Gs- CC specimens without cells, porosity was retained during incubation period. At all other cases, specimen's surface porosity was considerably decreased due to cell attachment and function and also due to sedimentation of medium's ingredients. EDS analysis indicated the existence of organic materials. OD values confirmed that specimens were successfully loaded with cells. However, only in CH-G-CC specimens human PDLF cells' proliferation rate was comparable with that of controls. On CHS-CC specimens the number of cells was progressively decreasing. Conclusions: Variations of cell proliferation rate and morphology were recorded on the examined specimens. These observations indicate that incubation in organic medium as well as in cells' culture influenced specimens' surface morphology, which in term affected cells' proliferation rate on CHS and CHS-G composite ceramics.