Potential CaP-based Treatment to Minimize Dentin Sensitivity and Bacterial Colonization

The aging population generally experiences gum recession that increases susceptibility to dentin sensitivity DS) and root caries. Strategies to treat DS are based on occluding the dentin tubules and strategies to prevent or minimize root caries are based on the use of fluoride and/or antibacterial agents. Objective: This study aims to develop a treatment that would address both occluding the dentin tubules and preventing bacterial adhesion and colonization. Methods: Dentin sections from caries free human molars were immersed for 1, 2, 4 minutes in acidic (pH5) saturated calcium phosphate solutions containing fluoride and zinc ions. The surfaces were characterized using SEM, EDS, X-ray diffraction, and FT-IR spectroscopy. Dissolution properties were determined in acidic buffer (pH5, 0.1M KAc, 37C). Anti-bacterial property was determined using Strep mutants (strain ATCC 25175). Results: Dentin tubule occlusions were observed on treated dentine, the percent of occluded dentin increasing with treatment time. The occlusions remained even after acid exposure. Treated dentin surfaces showed less extent of dissolution compared to the untreated surfaces. Significantly less bacterial colonization was observed on the treated surfaces. Conclusions: Results of this study suggest that the experimental CaP-based treatment has the potential to minimize dentin sensitivity (by occluding dentin tubules), provide a more acid-resistant and anti-bacterial property. [Supported by Calcium Research Funds].