The Cytotoxicity of Temporary Cements in vitro

Objectives: This study evaluated the cytotoxicity of eugenol-containing and eugenol-free temporary cements. Methods: For cytotoxicity testing, Bovine pulp-derived cells were exposed to extracts of eugenol-containing (Rely X Temp E) eugenol-free (Provicol, PreVision, Rely X Temp NE) temporary cements for 24 h. Extracts of specimens were prepared following the recommendations of ISO 10993-12. The cytotoxicity of the same materials was also analyzed in a dentin barrier test device using 3-D cell culture of Bovine pulp–derived cells. Cell viability was determined by dimethylthiazolediphenyltetrazolium bromide (MTT) assay. Results: The results revealed that cell survival with the extracts of Rely X Temp E, Provicol, PreVision and Rely X Temp NE was 89,17%, 84,97%, 92,30% and 66,87 % respectively. Extracts from Rely X Temp NE was cytotoxic to Bovine pulp-derived cells. (p < .05). The results of dentin barrier test revealed that cell survival with the above-mentioned temporary cement was 101%, 91%, 93% and 90% respectively. Statistically only Rely X Temp NE was cytotoxic when compared with control material (p < .05). Conclusion: Rely X Temp NE presented the most cytopathic effects to the bovine dental papilla derived cells. The attention should be noted in cases where the remaining dentin above pulp tissue is 0.5 mm or less when using Rely X Temp NE .