IADR Abstract Archives

Peptide profiling of parotid saliva in healthy and irradiated subjects

Objectives: Treatment of a tumour in the head and neck area often consists of irradiation with a high dose. Irradiation in this area can cause damage to the salivary glands which often results in hyposalivation. Hyposalivation can lead to the detoriation of the remaining dentition (rampant caries). This process could be aggravated by the loss of protective proteins from the saliva. It is however unclear whether protein changes in saliva occur that contribute to the loss of protective capacities of saliva. The aim of this study was to investigate differences in protein/peptide profiles of parotid saliva in irradiated patients and healthy controls.

Methods: Using Lashley cups, saliva of the left and right parotid gland was obtained from ten patients that received curative irradiation in the head and neck area and ten healthy controls. Salivary flow was stimulated using a 4% solution of citric acid during four minutes. After addition of a proteinase inhibitor cocktail, the samples were frozen in liquid nitrogen and stored at -80°C. The samples were analyzed with SELDI-TOF-MS using a NP20 and an IMAC-30 chip with values of the m/z ratio ranging from 1 to 30.000 kDa. Results: Comparison of the parotid saliva of irradiated patients and healthy controls showed 35 (out of 218) significantly different (up- or down-regulated) peaks using the IMAC-30 chip (p<0.01). The NP20 chip showed 69 (out of 217) significantly different (up- or down-regulated) peaks (p<0.01). On both chips, the significant different peaks presented troughout the range of m/z values. Significantly different peaks are, among others, in the range of the m/z ratio's of PRP's, histatins and statherin.

Conclusion: Besides a reduction in the amount of saliva, irradiation can cause changes in protein/peptide profiles that may be responsible for reduced protective properties of saliva. PRP's, histatins and statherin could be accountable for that.


IADR/PER General Session
2010 IADR/PER General Session (Barcelona, Spain)
Barcelona, Spain
2010
169
Salivary Research
  • Laheij, Alexa  ( Academic Centre for Dentistry Amsterdam, University of Amsterdam and Free University, Amsterdam, N/A, Netherlands )
  • Rasch, Coen  ( The Netherlands Cancer Institute, Amsterdam, N/A, Netherlands )
  • Schipper, Raymond  ( TI Food and Nutrition, Wageningen, N/A, Netherlands )
  • Loof, Arnoud  ( University Medical Centre St. Radboud, Nijmegen, N/A, Netherlands )
  • Silletti, Erika  ( TI Food and Nutrition, Wageningen, N/A, Netherlands )
  • Van Loveren, Cor  ( Academic Centre for Dentistry Amsterdam, University of Amsterdam and Free University, Amsterdam, N/A, Netherlands )
    • Oral Session
    Salivary Diagnostics
    07/14/2010