Effect Of Chemomechanical Material Used For Caries Removal In Pulp

Objectives: This study evaluates the cytotoxic effect of chemomechanical caries removal materials on human dental pulp fibroblasts, and its action on type I and III collagen, fibronectin and osteonectin immunolabeling. Methods: Three groups were included: Control; Papacárie® and Carisolv™. Cytotoxicity analysis was performed using conditioned medium on dental pulp fibroblast cells (FP1) after 0, 4, 8, and 12-hour exposure (short term) and 1, 3, 5, and 7-day exposure (long term). Proteins were searched by immunofluorescence. Results: Cell viability did not differ between the groups. The cell survival of the treated groups was significantly lower than that of the control after three days.All groups showed immunolabeling to vimentin, type I collagen, fibronectin andosteonectin. The osteonectin staining showed a weakly positive reaction in the control group, compared with the other groups. Conclusion: Papacárie® and Carisolv™ are not cytotoxic; however, they stimulate osteonectin production by these cells, probably leading to a more abundant dentin matrix formation.