Influence of Anatase-Coated Titanium on Dental Biofilm Formation in vivo
Objectives: Dental biofilms on transgingival implant surfaces might lead to inflammation of the surrounding tissues and to periimplantitis. Surface characteristics of implant materials affect the intraoral microbial colonization of implants. Titanium is commonly used as biocompatible implant material. Induced by UV-radiation nanocrystalline anatase-coating of titanium shows photoswitching from hydrophobic to strong hydrophilic surface conditions. The aim of this pilot study was to investigate the influence of UV-activated anatase-coated titanium on dental biofilm formation in vivo.
Methods: Six subjects wore intraoral stents supplied with UV-activated anatase-coated titanium samples (A-UV), anatase-coated titanium (A), titanium (Ti) and standardized human enamel (E) slivers serving as adhesion substrata for in vivo generation of biofilms (24h). Thereafter biofilm samples were labelled fluorescently using a LIVE/DEAD-stain and investigated in situ by CLSM. Microbial vitality (V) in different biofilm layers, biofilm thickness (BT) and surface area of bacterial colonization (C) were investigated. Mean values and corresponding 95% confidence intervals were calculated.
Results: Dental biofilm formation on all adhesion substrata showed interindividual variations. Biofilms had the highest V (72%) on A-UV and the lowest on Ti (46%). The inner biofilm layers adjacent to the adhesion substrata showed corresponding results. Mean BT ranged between 49µm (A-UV), 40µm (A), 35µm (E) and 30µm (Ti). 73% of the surface was colonized on A-UV compared to 52% (E), 47% (Ti) and 40% (A).
Conclusion: Dental biofilm formation was influenced by the different titanium surface modifications and conditions. The UV-activated strong hydrophilic surfaces (A-UV) seemed to enhance microbial vitality and colonization in vivo.
Acknowledgements: Supported by the Landesstiftung Baden-Württemberg
Division: IADR/PER General Session
Meeting:2010 IADR/PER General Session (Barcelona, Spain) Location: Barcelona, Spain
Year: 2010 Final Presentation ID:4543 Abstract Category|Abstract Category(s):Dental Materials 5: Biocompatibility and Biologic Effects
Authors
Von Ohle, Christiane
( University of Tuebingen, Tuebingen, N/A, Germany
)
Geis-gerstorfer, Jurgen
( University of Tuebingen, Tuebingen, N/A, Germany
)
Decker, Eva-maria
( University of Tuebingen, Tuebingen, N/A, Germany
)
Camillo-carrascoza, Karina
( University of Tuebingen, Tuebingen, N/A, Germany
)
Haupt, Michael
( Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Stuttgart, N/A, Germany
)
Oehr, Christian
( Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Stuttgart, N/A, Germany
)
Rupp, Frank
( University of Tuebingen, Tuebingen, N/A, Germany
)
Scheideler, Lutz
( University of Tuebingen, Tuebingen, N/A, Germany
)
Sinn, Stefan
( University of Tuebingen, Tuebingen, N/A, Germany
)
Wendel, Hp
( University of Tuebingen, Tuebingen, N/A, Germany
)
SESSION INFORMATION
Poster Session
Biocompatibility and Biologic Effects IV
07/17/2010