Objectives: Although newly developed calcium phosphate based sealer (CAPSEAL I and II) showed superior properties in anaerobic bacterial leakage model and lower tissue response in rat, its effects on cultured human periodontal ligament cells (HPDLCs) was not reported. The aim of this study was to investigate the cellular effects of CAPSEAL I and II such as cytotoxity, osteoblastic differentiation and inflammatory mediators on cultured HPDLCs, compared with epoxy resin sealer (AH26), ZOE sealer (EWT) and CPC sealer (Sankin). Methods: Cell viability by MTT assay, cell attachment by SEM, differentiation and inflammatory mediators by RT-PCR, Western-blot and alizarin red staining were evaluated on CAPSEAL I, CAPSEAL II, AH26, EWT and Sankin. Results: The cytotoxicity of CAPSEAL ± and II were less than AH 26 and EWT for 1, 3, 7 and 14 days. HPDLCs on CAPSEAL I and II were spread better compared with those on other sealers. Mineralization and expression of osteoblastic differentiation markers such as alkaline phosphate and osteonectin mRNA were increased in CAPSEAL I and II exposed HPDLCs for 1 and 3 days. Production of inflammatory mediators including COX-2 and iNOS and ROS were lower in CAPSEAL I and II compared with control and other sealers. Conclusion: These results suggested that both CAPSEAL I and II show less cytotoxity and inflammatory mediators, and have potential to promote bone regeneration as root canal sealers.
ACKNOWLEDGEMENT; This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family affairs, Republic of Korea (A084458).