Altering the surface properties of acrylic resin may influence microbial adherence and prevent denture-related stomatitis. Objectives: the aim of this study was to assess the in vitro adherence of C. albicans to acrylic resin surfaces treated with glow discharge plasma, and to observe the effect of salivary coating on candidal adhesion. Methods: disks (13.8x2 mm) were fabricated with a microwave-polymerized acrylic resin (VIPI WAVE) processed against stone to simulate the unpolished fitting surface of the dentures. Specimens were divided into two groups (n=12): G1-no treatment (control); and G2-treatment with plasma under Ar and SF6 gas (pressure: 10-1Torr; discharge power: 70W; exposure time: 5min). Water contact angles (WCA) of all surfaces were measured using a goniometer. Surface composition of the specimens was analyzed with x-ray photoelectron spectroscopic (XPS). Half of the specimens were coated with unstimulated whole saliva for 30min. All specimens were placed in C. albicans (ATCC 90028) suspensions (107cells/ml) and incubated for 90min at 37°C. The number of adherent cells was determined by the XTT-reduction assay, which was performed in triplicate in two independent experiments. Data from WCA measurements and XTT were analyzed by ANOVA test (a=0.05). Results: the mean WCA of G1 (59.23º) and G2 (60.17º) did not differ significantly. XPS spectra of plasma-treated specimens showed the presence of fluorine atoms. No significant difference was observed in the mean number of adherent cells between G1 (0.470) and G2 (0.490), and saliva pre-conditioning had no significant effect on the initial attachment of C. albicans. XPS analysis showed that glow discharge plasma altered the surface of the acrylic resin, but had no influence on the mean WCA after water imersion. Conclusions: the adhesion of C. albicans to the denture base acrylic resin evaluated was not affected by either the plasma treatment or the saliva pellicle.