Cytotoxicity of three perforation repair materials on HPLC in vitro

Objective: To evaluation the cytotoxicity of three kinds of perforation repair materials on human periodontal ligament fibroblasts( HPDLF)in vitro. Methods: The proliferation of HPDLFs to three perforation repair materials were examined by MTT-assay at days 1, 3 and 5 days,The mRNA expression levels of bone-associated AKP and OC were determined using a real-time quantitative RT-PCR at 5 days. RESULTS: MTA shew almost no inhibition to HPDLFs and was grade 1, quantitative RT-PCR revealed higher mRNA expression of AKP and OC in the HPDLFs cultured on MTA; Z350 induced a slight inhibition to HPDLFs, and Z350 induced the mRNA expression of AKP but there was no difference in the mRNA expression of OC; cell proliferation was significantly impaired by amalgam with grade 3, and the mRNA expression of AKP and OC were significantly reduced. CONCLUSION: MTA had minimum cytotoxicity on HPDLFs and could promote cell differentiation and regenerate of periodontal tissue; Z350 had lower cytotoxicity on HPDLFs; amalgam shew highest cytotoxicity on HPDLFs in the three materials and inhibited cells differentiation.