Biodegradable scaffolds and tissue reactions a pilot study in mice
Objectives: Bone and soft tissue regeneration constitute research areas of high interest. Aiming at a material with properties satisfactory for regenerative bone and periodontal therapies, new co-polymer scaffolds have been developed for tissue engineering. We have recently shown that two porous scaffolds synthesized by bulk copolymerization of poly (L-lactide) (PLLA) and 1,5-dioxepan-2-one (DXO-co-LLA), or є-caprolactone (CL) and L-lactide monomer (CL-co-LLA) have good properties for cell growth in vitro. The aim of this study was to determine the inflammatory reaction to DXO-co-LLA and CL-co-LLA as well as blood vessel formation in the scaffolds, when placed 6 weeks in a subcutaneous mice model.
Methods: Six weeks old female BALB/cJBomTac mice were used. During Isoflurane sedation, two scaffolds, (pore sizes <90µm and >90µm, DXO-co-LLA or CL-co-LLA) were placed subcutaneously at the flanks of the back. After six weeks the mice were euthanized, and the scaffolds removed together with the surrounding tissues. Samples were immersed in Histocon for 24h and frozen in Tissue-Tek at -80ºC. Frozen sections of 8µm were studied by Hematoxylin-Eosin and immunohistochemistry for CD 31.
Results: No or sparse inflammatory reactions in the tissues surrounding the scaffolds were detected. Connective tissue cells grew inside the two types of scaffolds. Scaffolds with pore size >90µm showed more cell growth inside the material, and demonstrated signs of scaffold degradation. The formation of new blood vessels could also be detected inside the both scaffolds.
Conclusion: Our study shows that the materials full-fill some of the basic requirements for a scaffold material, e.g. allow cells to adhere and migrate, cause little or no inflammatory reaction, the inter-connecting pores allow cell- and tissue- growth, and blood vessel formation.
This study was supported by Helse Vest and the Norwegian Research Council
IADR/CADR General Session
2008 IADR/CADR General Session (Toronto, Ontario, Canada) Toronto, Ontario, Canada
2008 56 Implantology Research
Schander, Kerstin Elisabeth
( Faculty of Medicine and Dentistry, University of Bergen, Bergen, N/A, Norway
)
Mustafa, Kamal
( Faculty of Medicine and Dentistry, University of Bergen, Bergen, N/A, Norway
)
Hellem, Endre
( Faculty of Medicine and Dentistry, University of Bergen, Bergen, N/A, Norway
)
Bolstad, Anne Isine
( Faculty of Medicine and Dentistry, University of Bergen, Bergen, N/A, Norway
)
Arvidson, Kristina
( Faculty of Medicine and Dentistry, University of Bergen, Bergen, N/A, Norway
)
Hammer Østvold, Siren
( Faculty of Medicine and Dentistry, University of Bergen, Bergen, N/A, Norway
)
Dånmark, Staffan
( Faculty of Medicine and Dentistry, University of Bergen, Bergen, N/A, Norway
)
Albertsson, Ann-christine
( Royal Institute of Technology, Stockholm, N/A, Sweden
)
Plikk, Peter
( Royal Institute of Technology, Stockholm, N/A, Sweden
)
Wistrand, Anna
( Royal Institute of Technology, Stockholm, N/A, Sweden
)
