IADR Abstract Archives
Mast Cells – Important in TMD and TMJ Implant Host Response?
Mast Cells Ð Important in TMD and TMJ Implant Host Response?
Objectives: Mast cells (MCs) are an important component of inflammation and their contributions to allergic responses are well documented. Limited studies have demonstrated the presence of MCs in the temporomandibular joint (TMJ). It is not known if they participate directly in temporomandibular disorders (TMD) or TMJ host response to implant wear debris. The goal of this pilot project is to identify and count MCs in tissues from subjects with TMD and TMJ implant wear debris [Proplast-teflon¨ or Silastic¨]. Methods: Three groups were selected. I. Tissues with implant wear debris (n=5), II. Tissues without implant wear debris (n=5), and III. Cadaver control tissues with no history of TMD or TMJ surgery (n=5). Immunohistochemical (IHC) staining was performed to detect mast cells using monoclonal antibody, Mast Cell Tryptase (clone AA1) (Dako, Carpinteria, CA). MCs were counted in tissue sections at 40X (Olympus BX50 microscope) using a Hand Tally counter (Fisher Scientific, Pittsburgh, PA). Results: All IHC positive staining cells were tallied and counts averaged and statistically analyzed using one-way fixed ANOVA. No statistical difference in mast cell counts between the three groups was detected. Results are shown in Table 1. Table 1: Mean Number of Mast Cells in Tissue Sections
|
Study Groups |
Mean Count/sq mm |
SE |
|
Group I* (n=5) |
3.15 |
1.22 |
|
Group II** (n=5) |
1.62 |
0.7 |
|
Group III*** (n=5) |
0.72 |
0.24 |
|
F value=2.21; p=0.15 |
||
*Implant
**Non-implant
***Control
Conclusion: Preliminary results suggest that mast cells may not participate directly in TMD or implant-related tissue reactions since they did not vary significantly between the three groups. Further investigations will help characterize the direct and indirect roles of mast cells in TMJ inflammatory response. Funded by U of M OHCRC and supported in part by NIDCR's TIRR/NO1-DE-22635.