Influence of Desenitizers and Adhesives on Dentin Demineralization in Situ

Objectives: The objective of this study was to evaluate the influence of five bonding agents and desensitizers on dentin demineralization in situ. Methods: The root surfaces of 60 freshly extracted human molars were thoroughly cleaned, thereby removing the cementum. From each tooth root dentin specimens were prepared. 120 specimens were distributed among the following experimental groups: A: Syntac, B: Xeno III, C: Admira Protect, D: Gluma Desensitizrer, E: Hyposen, F: control group, untreated. Two dentin specimens of each group were inserted into two buccal aspects of ten intraoral mandibular appliances. The appliances were worn by ten persons for five weeks. One side was brushed daily with a fluoride-containing toothpaste (Aronal). During meals, the appliance was stored in 10% sucrose solution. After the in situ period, two slabs (150 microns) were ground. The depth of the demineralized areas was determined using a polarized light microscope. For each group mean value and standard deviation were calculated. Statistical analysis was performed using ANOVA and Tukeys test. Results: Concerning lesion depth, ANOVA revealed significant differences between brushed (b) and non-brushed (nb) specimens. Following lesion depths were evaluated (mean values and standard deviation in microns):

Compared to the untreated control, lesion depths in groups A-E were significantly decreased in the brushed and also in the non-brushed subgroups (p<0.05, Tukeys test). Conclusions: Within the limitations of an in situ study it can be concluded that the demineralization of the root surface can be hampered by application of dentin adhesives and desensitizers under different oral hygiene conditions.