In degenerative temporomandibular joint (TMJ) disorders, there is a breakdown of the collagen containing structures of the TMJ. The matrix metalloproteinases (MMPs) expressed by TMJ synovial fibroblasts are believed to play a role in regulating this collagen turnover. Cytokine expression has been shown to regulate the expression of these MMPs. Recently, a TMJ synovial fibroblast cell line at early passages was shown to be capable of degrading collagen utilizing just a MMP-dependent pathway and is referred to as the non-aggressive phenotype. However, this cell line after about 8 passages developed the ability to cleave collagen more aggressively by utilizing both a MMP-dependent and a MMP-independent pathway and is referred to as the aggressive phenotype. Objectives: The purpose of this study was to examine the cytokine and MMP protein expression of the non-aggressive and aggressive phenotypes. Methods: Conditioned media from the non-aggressive and the aggressive phenotypes was used to identify the cytokines expressed utilizing RayBio® Human Cytokine Antibody Arrays. Western blot analyses were also performed to examine the presence of select MMPs secreted into the conditioned media. Results: The cytokine arrays demonstrated that both the non-aggressive and the aggressive cells expressed IL-6, IL-8, and MCP-1. Low levels of GRO and TNF-β were also expressed by both phenotypes. The aggressive cells also expressed GCSF, whereas the non-aggressive also expressed GRO-α, IFN-γ, MCP-2, RANTES, and TGF-β1. Western blot analyses detected MMP-1, MMP-2, MMP-3, and MMP-9 in the conditioned media from both the non-aggressive and aggressive cells. Conclusion: The lack of a drastic difference in MMP expression further suggests that the ability of the aggressive phenotype to cleave collagen is not dependent on altered MMP expression. The differences in cytokine expression could contribute indirectly to the increased collagen degradation observed in the aggressive phenotype.