Saliva Contaminated One-bottle Adhesive: Effect of Decontamination with Phosphoric acid

Objectives: The aims of this study were to investigate the effect of saliva contamination in different steps of using a one-bottle adhesive, called i-Bond, and using phosphoric acid after salivary contamination of enamel on bonding efficacy of the adhesive system. Methods: Eighty-four recently extracted human premolars were prepared to obtain fresh enamel surfaces, randomly separated into 7 groups(n=12). Group 1 specimens(positive control group) were not contaminated, the bonding system, i-Bond(Heraeus-kulzer,Germany), was applied according to manufacturer's instructions. In groups 2-4 samples were contaminated with fresh human saliva(30s ) prior to applying and curing the adhesive. In groups 2 and 3 adhesive applied without and with rinsing of saliva respectively. In specimens of group 4 after rinsing(15S ), 37% phosphoric acid applied(10S ), rinsed(15S ) and blot dried(5S ). In groups 5-7, contamination occurred after adhesive curing. Group 5: only rinsed, group 6: rinsed, adhesive reapplied, group 7: rinsed, phosphoric acid used, rinsed and adhesive reapplied. Cylinders of composite applied and light cured. Specimens mounted and tested in shear in a DARTEC testing machine. Data were analyzed using one-way ANOVA and t-test analyses at the P=0/05 level of significant difference between group means. Results: Mean bond strengths values for groups were: 17.409,12.514,15.333,18.459,13.069,10.368,12.267 Mpa respectively. There were statistically significant differences between group means. Groups 1 and 4 demonstrated higher bond strength than other groups. Among the groups, 1 and 2 and 5-7, 2 and 4 and 5-7 demonstrated significant differences respectively. Conclusion: Saliva contamination did affect the shear bond strength of i-Bond. Using phosphoric acid may be effective, provided that the contamination occur prior to curing of the adhesive. In the other hand, when salivary contamination occurs prior to curing of i-Bond system using of phosphoric acid may be suggested. After curing of adhesive, simple rinsing of saliva would be preferred.