Effects of Silver Staining on the Adhesion to Dentin

Background: Silver nitrate staining is a reliable technique to trace out the nanoleakage or microleakage in adhesion interface between dentin and adhesive.Objectives:To  evaluate the effect of silver nitrate staining on the microtensile bond strengths of three adhesives to normal versus caries-affected dentin (CA dentin).Methods:30 extracted human molars with coronal carious lesions invading mid-dentin were selected and were ground down by means of 600-grit SiC paper until the carious dentin no longer stained with caries detector (Caries Marker, VOCO).The flat  surfaces were bonded with either Prime&Bond NT (Dentsply), Adper Prompt (3M), or Contax (DMG), according to manufacturers' recommendations.A crown was built up using composite (Chrisma).After storage in water (37^ºC, 24 h), teeth were vertically serially sectioned into 0.7 mm thick slabs and trimmed to yield 1 mm²  test area that contained either normal or CA dentin. Samples were immersed in water (control) or ammoniac silver nitrate solution for 8 hours, and then  were tested in tension at a speed of 1 mm/min.Results:  No significant differences (P>0.05) in bond strengths were found between water and silver nitrate storage groups in three adhesives.

Microtensile bond strengths (X±SD, in MPa).

Conclusions: Silver nitrate staining has no effect on the microtensile bond strengths of three adhesives to normal versus caries-affected dentin. This study was supported by National Natural Science Foundation of China (#30470433).