Objectives: The purpose of this study was to quantify and compare the level of IL-1b, MMP-13 and TIMP-1 in the healthy, inflammed gingival tissue and inflammed gingival tissue associated with type 2 DM. Methods: Gingival tissue samples were obtained during periodontal surgery or tooth extraction. According to the patient's systemic condition & clinical criteria of gingiva, each gingival sample was devided into three groups. Group 1 (n=8) is clinically healthy gingiva without bleeding and no evidence of bone resorption or periodontal pockets, obtained from systemically healthy 8 patients. Group 2 (n=8) is inflammed gingiva from patients with chronic periodontitis. Group 3 (n=8) is inflammed gingiva from patients with chronic periodontitis associated with type 2 diabetes. Tissue samples were prepared and analyzed by Western blotting. The quantification of IL-1b, MMP-13, TIMP-1 were performed using a densitometer and statistically analyzed by one-way ANOVA followed by Tukey test. Results: The expressions of MMP-13 and TIMP-1 showed increasing tendency in group 2 & 3 compared to group 1. The expressions of IL-1b & MMP- 13 showed increasing tendency in group 3 compared to group 2. As IL-1b levels were increasing, MMP-13 was increasing tendency in group 3, and although IL-1b, MMP-13 levels were increasing, TIMP-1 levels were similar expression comparing to group 2. Conclusion: this study demonstrated that the expression levels of MMP-13 and TIMP-1 had increasing tendency in inflammed tissue. It can be assumed that IL-1b, MMP-13 and TIMP-1 may be partly involved in the progression of periodontal inflammation associated to type 2 DM.