In vivo study of injectable PLA/Chitosan GTR barrier

The improvement in surgical technique and barrier materials has made the GTR technology successfully applied in treating periodontal diseases. A variety of bioabsorbable barrier materials have been marketed there few years, offering the benefit of no need for second-stage surgery. A resorbable PLA/Chitosan barrier functioned as a guided tissue regeneration membrane by protecting the surgical site from the in growth of epithelium cell was developed in our laboratory. Advantages of reducing the bacterial colonization of the barrier but without antibiotics addition were implemented in this GTR barrier system to meet specific human biological requirements and to reduce duration of healing. Objectives: The goal of this in vivo study is to evaluate the efficacy of this PLA/Chitosan GTR barrier with antibacterial properties. Materials and methods: 4 x 5 mm 2-wall intrabony defects were surgically created in the bilateral mandibular first, third premolars and fourth premolars and second molars in 6 beagle dogs. The 1st group was treated by DFDBA, while 2nd group were treated by DFDBA + Atrisorb®, and 3rd group were treated by DFDBA + PLA/Chitosan membrane. Tested dogs were sacrificed 1 week, 4 weeks and 12 weeks after operation. Result: Sites treated with DFDBA alone presented a lesser bone area compared to DFDBA + Atrisorb® and DFDBA + PLA/Chitosan. No statistically significant difference was found between Atrisorb® and PLA/Chitosan. Conclusion: The results demonstrate the beneficial effect of the PLA/Chitosan membrane on the two-wall intrabony defect of beagle dogs. Updated information concerning the factors that influence the success of GTR membranes and the further research directions will be obtained.