IADR Abstract Archives

Cariostatic Effect of Extracts of Nidus Vespae on Oral Bacteria

Introduction Nidus Vespae, a natural beehive product, was mainly composed of polyphenolic compounds, such as flavonoids and cinnamic acid derivatives. Our previous study demonstrated it has anticaries effect. Objective: The aim of this study was to investigate the role of different components of Nidus Vespae extract on the growth and acid production of 6 kinds of cariogenic bacteria, and to find out the most effective components of Nidus Vespae extract. Methods: Four different components(NVE1,NVE2, NVE3,NVE4) were separated from Nidus Vespae and there antibacterial activities to Streptococcus mutans ATCC 25175, Streptococcus sanguis ATCC 10556, Streptococcus salivarius SS 196, Actinomyces naeslundii WVU 627, Actinomyces viscosus ATCC 19246 and Lactobacillus rhamnosus AC 413 were checked. There effects on the growth curve of Streptococcus mutans ATCC 25175 were also investigated. According to the MIC values of each component, the most effective components were checked for acid production test. Acid production from sucrose was assessed with a standard pH meter (Orion 710A). Results: The most effective part of Nidus Vespae were found to be NVE1,NVE2 and NVE3, which were found to be a mixture of sterols. and NVE3 have an inhibitory effect to the growth of Streptococcus mutans ATCC 25175. For the acid production assay, NVE1 and NVE2 could inhibit the acid production of S. sanguis, S.salivarius, A.naeslundii, A.viscosus and L.rhamnosus more effectively than other extract of Galla Chinesis with significant difference. Conclusion: NVE1 and NVE2 could inhibit the growth of the tested bacteria effectively. These results suggest that the antibacterial activity of Nidus Vespae extract is caused by a synergistic effect of sterols. Support for this study is provided from Chinese Natural Science Grant (Grant No.30171175)


IADR/AADR/CADR General Session
2005 IADR/AADR/CADR General Session (Baltimore, Maryland)
Baltimore, Maryland
2005
3433
Microbiology / Immunology and Infection Control
  • Zuo, Yuling  ( Sichuan University, Chengdu, N/A, China )
  • Xie, Qian  ( Sichuan University, Chengdu, N/A, China )
  • Zhu, Bing  ( Sichuan University, Chengdu, N/A, China )
  • Zhao, Jing  ( Sichuan University, Chengdu, N/A, China )
  • Li, Jiyao  ( Sichuan University, Chengdu, N/A, China )
  • Zhou, Xuedong  ( Sichuan University, Chengdu, N/A, China )
  • Poster Session
    Candida
    03/12/2005