Methods: The PRP was obtained from a human source. Two experiments were conducted. In the first one, PRP was diluted to 50, 25, 12.5 and 6.125 (v/v) with culture medium (MEM:HAM F12 and 1% antibiotics-antimicotic) supplemented with 10% of fetal bovine serum (FBS). In the second experiment, all conditions were identical except for the absence of FBS in the culture medium. To visualize the osteoblasts nuclei activity an epyfluorescent microscopy was used, and the obtained results showed a high nuclei activity and defined cell extensions.
Results: The results of the osteoblasts proliferation test were higher when stimulated by the 50% PRP dilution with or without FBS. A further study is suggested to determine if concentrations above 50% could cause higher rates of osteoblasts proliferation. In this study, the results were not statistically different (p<0.05) with 12.5 and 6.125% PRP dilutions. Additionally, it was shown that FBS is not necessary for PRP mediated induction of osteoblasts proliferation. DAPI staining permited visualization of hFOB1.19 osteoblasts which showed well defined active nuclei and wide cytoplasm (Fig. 2)
Conclusion: This study concluded that PRP promotes osteoblasts proliferation and suggests its clinical application to bone graft procedures in implant dentistry.