Effect of PLA on Biological Behavior of Rabbit MSC

Skeletal defect from all kinds of reasons is an ordinary but difficult disease. Tissue engineering technology is more exciting in the efforts to solve the problem contrast to traditional methods. Biodegradable scaffolding materials and bone marrow stromal cells(MSC) have been evaluated in vivo and in vitro experiments. Objective: To investigate the effect of modified PLA scaffolds on biological behavior of rabbit MSC and the procedure for fabricating tissue-engineered bone. Methods: The proliferation and osteogenic differentiation of MSC was investigated in three-dimensional PLA, PLGA, PLA ¨CPEG, TCP (as a control), including the adhesion, cell proliferation, alkaline phosphatase activity, and synthesization of osteocalcin. 16 samples were included in every group. Results: MSC cells can attach to all scaffolds and grow normally, proliferate, function actively with PLGA the most and PLA the least. When the alkaline phosphatase (ALP) activity and osteocalcin content of MSC cultured in four different scaffolds was measured to evaluate the osteogenic differentiation, they became maximum for PLGA and minimum for PLA with significant difference. Conclusions: The MSC can adhere to these polymeric scaffolds and grow, proliferate and osteogenic differentiate actively, the cells spread better in PLGA group. All chosen scaffolds including biodegradable PLA, PLGA, PLA-PEG and TCP have outstanding osteoconductivity and biocompatibility within which PLGA is the best one.