Antibacterial Activity of Tanshinones from the Root of Salvia miltiorrhiza
Objective: Actinobacillus actinomycetemcomitans and methicillin-resistant Staphylococcus aureus has been emerging worldwide as one of the most important problems in dental and medical fields. Therefore, new agents are needed to treat the A. actinomycetemcomitans and MRSA. To investigate the antibacterial agents against A. actinomycetemcomitans ATCC 43717 and MRSA from oral infection, several natural product extracts were screened. The active compounds, tanshinones from Salvia miltiorrhiza were isolated successively through the screening system and various purification methods. Methods: S. miltiorrhiza was extracted with 80% EtOH. The extract was suspended in H2O and partitioned with CHCl3, EtOAC, and n-BuOH in sequence. The CHCl3 fraction showed remarkable antibacterial activity against A. actinomycetemcomitans and MRSA. Column chromatography of the most active fraction on silica gel and recycling prep-HPLC gave purified compounds. The structure of compounds was identified by MS, 1H-NMR, and 13C-NMR. Results: The CHCl3 fraction from solvent partition possessed greater antibacterial potential than other fractions. The fraction were obtained from column chromatography with silica gel had antibacterial activity against A. actinomycetemcomitans and MRSA, and the fraction was separated by recycling prep-HPLC to purified compounds. Their structures were identified as dihydrotanshinone ¥°, crytotanshinone, tanshinone¥°, and tanshinone ¥±A by MS, 1H-NMR, and 13C-NMR. The minimum inhibitory concentrations (MICs) of tanshinones to the A. actinomycetemcomitans and S. aureus (MRSA and MSSA) was 15 to 250 mM and 2 to 64 mM, respectively. Conclusion: These results suggest that tanshinones might have the antibacterial activity against A. actinomycetemcomitans and MRSA from acute oral infection. This paper was supported by a grant No. R01-2001-000-00133-0 from Korean Science & Engineering Foundation
Division: IADR/AADR/CADR General Session
Meeting:2004 IADR/AADR/CADR General Session (Honolulu, Hawaii) Location: Honolulu, Hawaii
Year: 2004 Final Presentation ID:106 Abstract Category|Abstract Category(s):Microbiology / Immunology and Infection Control
Authors
Shin, H.s.
( Wonkwang University, Chonbuk, N/A, South Korea
)
Jeong, S.i
( Wonkwang University, Chonbuk, N/A, South Korea
)
You, Y.-o.
( Wonkwang University, Chonbuk, N/A, South Korea
)
Lee, B.m.
( Wonkwang University, Chonbuk, N/A, South Korea
)
Yang, C.h.
( Wonkwang University, Chonbuk, N/A, South Korea
)
Lee, S.h.
( Wonkwang University, Chonbuk, N/A, South Korea
)
Han, S.h.
( Wonkwang University, Chonbuk, N/A, South Korea
)
Yu, H.h.
( Wonkwang University, Chonbuk, N/A, South Korea
)
Kim, K.j.
( Wonkwang University, Chonbuk, N/A, South Korea
)