Antibacterial Activity of Curcumin and Bisdimethyl-Curcumin Isolated from Curcuma longa
Objective: Actinobacillus actinomycetemcomitans and antibiotic-resistant bacteria infection is the one of important problem in dental and medical fields. Therefore, new agents are needed to treat Actinobacillus actinomycetemcomitans and methicillin-resistant Staphylococcus aureus from oral infection. In the present study, we investigated the antimicrobial activity of Curcuma longa and the antibacterial components, curcumin and bisdemethyl curcumin, from C. longa has been isolated and identified by MS, 1H-NMR, and 13C-NMR. Methods: C. longa was extracted with 80% MeOH, the extract was concentrated in vacua and the residue partitioned between H2O and CHCl3, EtOAc, and n-BuOH, respectively. The fractions of CHCl3 were assayed for growth inhibitory activity against A. actinomycetemcomitans and MRSA. Column chromatography of the most active fraction on silica gel, Sephadex LH20, and prep-HPLC gave purified compounds. The structure of compounds was identified by MS, 1H-NMR, and 13C-NMR. Results: The CHCl3 fraction from solvent partition possessed greater antibacterial potential than other fractions. The fractions were obtained from column chromatography with silica gel and Sephadex LH20 had antibacterial effect, and the fraction was separated by prep-HPLC to purified compounds. The compounds were identified as curcumin and bisdemethyl curcumin by MS, 1H-NMR, and 13C-NMR. They had antibacterial activity against the A. actinomycetemcomitans and MRSA. Conclusion: These results suggest that curcumin and bisdemethyl isolated from C. longa by bioactivity-guided fractionation may have antibacterial activity against A. actinomycetemcomitans and MRSA. This paper was supported by a grant No. R01-2001-000-00133-0 from Korean Science & Engineering Foundation.
Division: IADR/AADR/CADR General Session
Meeting:2004 IADR/AADR/CADR General Session (Honolulu, Hawaii) Location: Honolulu, Hawaii
Year: 2004 Final Presentation ID:105 Abstract Category|Abstract Category(s):Microbiology / Immunology and Infection Control
Authors
Kim, Kang-ju
( Wonkwang University, Chonbuk, N/A, South Korea
)
Jeong, S.i.
( Wonkwang University, Chonbuk, N/A, South Korea
)
Shin, H.s.
( Wonkwang University, Chonbuk, N/A, South Korea
)
Lee, B.m.
( Wonkwang University, Chonbuk, N/A, South Korea
)
Yang, C.h.
( Wonkwang University, Chonbuk, N/A, South Korea
)
Lee, S.h.
( Wonkwang University, Chonbuk, N/A, South Korea
)
Han, S.h.
( Wonkwang University, Chonbuk, N/A, South Korea
)
Yu, H.h.
( Wonkwang University, Chonbuk, N/A, South Korea
)
You, Y.-o.
( Wonkwang University, Chonbuk, N/A, South Korea
)