IADR Abstract Archives

Disinfecting Musical Instrument Source of Oral and Respiratory Disease Transmission

Objectives: Bagpipes are easily contaminated wind instruments it has a pouch reservoir made from sheepskin leather, or Gortex®. Wind to the instrument is introduced into the mouthpiece.  This blowing action introduces host aerosolized microorganisms normally resident in the oropharyngeal cavity and respiratory tract. The bag lends itself to an accumulation of potentially pathogenic microorganisms which can pose serious risks to main users.  The leather bags are more susceptible to contamination because of the use of “seasoning”, a greasy material intended to soften and seal the leather bag.  The synthetic bags are less susceptible to such contamination.  To reduce the risk of infection, the inner surface was disinfected with BioSURF. Methods:  Bagpipe pouch (Scott's Highland),  T-soy agar plates, BioSURF™ (Micrylium, Toronto).  The sealed bagpipe sac was prewashed with 500 ml of deionized sterilized water by shaking for 5 minutes allowing contact. The water was collected in a sterilized container. The bag then was treated with 500ml of disinfectant (BioSURFand then rinsed twice with 500 ml of de-ionized sterilized distilled water. Three different assays were performed, each on the prewash, BioSURF™ wash and postwash respectively. Assay:  Each treatment was serially diluted 101-106 in water and 100 μl. of each eluent, that is:  The dilutions were plated on T-soy agar and incubated at 37o C for 48 hours.  The colonies were counted manually and converted to colony forming units. Results:  The results showed a pre-disinfection contamination with > 109 cfu., which when diluted did not decrease significantly until the 4th dilution at 104.  The test rinse and post test harvest showed no bacterial growth. Conclusions: The results show that BioSURF™ in the presence of a high bacterial load and protected by a lanolin high organic layer was able to reduce bacterial counts within the sac to zero within 5 minutes.


Division: IADR/AADR/CADR General Session
Meeting: 2004 IADR/AADR/CADR General Session (Honolulu, Hawaii)
Location: Honolulu, Hawaii
Year: 2004
Final Presentation ID: 2245
Abstract Category|Abstract Category(s): Microbiology / Immunology and Infection Control
Authors
  • Traynor, Theresa  ( Micrylium Laboratories Inc, Toronto, ON, Canada )
  • Maharaj, Indar  ( Micrylium Laboratories Inc, Toronto, ON, Canada )
  • Kalupnieks, Andris  ( Micrylium Laboratories Inc, Toronto, ON, Canada )
  • Swift, Dean  ( Micrylium Laboratories Inc, Toronto, ON, Canada )
  • SESSION INFORMATION
    Poster Session
    Disinfection and Infection Control
    03/12/2004