Objective: Evaluate, morphologically and tracking the presence of elastin, if the PC-E matrices are being partially incorporated into bone extracellular matrix.
Methods: Samples of PC-Es were implanted into 4 mm bone defects surgically created in rats. The structures were harvested 15, 30 and 60 days after implantation and analyzed by means of hematoxilyn & eosin and Weigert’s resorcin-fucsin stain for elastic fibers in an upright light microscope.
Results: Fifteen days post implantation the porous structure of the three matrices was filled with mesenquimal cells. Small amounts of newly formed bone could be distinguished at the periphery and the core of the implants. Elastin was revealed along the structure of all matrices and within the structure of the new formed bone. By the thirtieth day extensive areas of woven bone could be seen where previously the matrices were placed. The collagen organization in this tissue was similar to the implants and numerous elastic fibers were entrapped in this tissue. Sixty days after surgery the morphological aspect was similar to the one observed 30 days before, except for the greater amount of mature bone. At any period direct resorption of the matrices by inflammatory cells could be seen.
Conclusion: PC-Es can be regarded as real provisional matrix, especially for woven bone, possibly taking an active part in the process of matrix mineralization.
Financial support: FAPESP(Proc.: 01/05634-0)