Distribution of Human Lactoferrin on the Periodontally Affected Dental Root
Objective: Human lactoferrin (hLf) is an
iron-binding protein found in milk, tears and neutrophils. A recent
study suggested that hLf inhibits lipopolysaccharide (LPS)
interaction with CD14. LPS from periodontopathic bacteria permeates
dental root surfaces exposed to periodontal pockets, eliciting an
inflammatory response. Thus, hLf released from migrating neutrophils
may be involved in the inflammatory response. The objective of the
present study was to investigate the distribution of hLf on dental
roots. Methods: Teeth not salvageable by periodontic treatment
were extracted with the informed consent of patients. Powdery samples
were prepared from extracted roots with a round bur-equipped electric
motor-driven system. The samples were collected and partitioned into
three groups on the basis of location: Layers I, II and III (from the
root surface to the pulp cavity). Specimens were eluted in PBS.
Following centrifugation, the supernatant was used to measure hLf
levels. Subsequently, extracted teeth were incubated with anti-hLf
antibody and Alexa 532-conjugated secondary antibody. Fluorescence
measurements were performed on a Molecular Imager FX Pro¨. In
addition, hLf was introduced to J774.1 murine macrophage-like cells
infected with A. actinomycetemcomitans in order to evaluate
the effects on apoptotis. Results: Significantly higher hLf
levels were detected in Layer I from the area with calculus in
comparison to Layer I from the area without calculus. hLf levels
decreased from Layer I to Layer III. The area displaying fluorescence
was in agreement with the area with calculus. LDH activity, DNA
fragmentation and TNF-a
production in the infected J774.1 cells were reduced upon addition of
hLf. Conclusion: These findings suggested that hLf released by
neutrophils is bound to LPS leading to inhibition of the
immunobiological effects of LPS to host cells, which results in host
protection.
Division: AADR/CADR Annual Meeting
Meeting:2003 AADR/CADR Annual Meeting (San Antonio, Texas) Location: San Antonio, Texas
Year: 2003 Final Presentation ID:1433 Abstract Category|Abstract Category(s):Periodontal Research - Pathogenesis
Authors
Nakashima, Keisuke
( Health Sciences University of Hokkaido, Ishikari-Tobetsu, N/A, Japan
)
Fujiwara, Jun
( Health Sciences University of Hokkaido, Ishikari-Tobetsu, N/A, Japan
)
Kobayashi, Takao
( Health Sciences University of Hokkaido, Ishikari-Tobetsu, N/A, Japan
)
Tomioka, Jun
( Health Sciences University of Hokkaido, Ishikari-Tobetsu, N/A, Japan
)
Kato, Satsuki
( Health Sciences University of Hokkaido, Ishikari-Tobetsu, N/A, Japan
)
Kowashi, Yusuke
( Health Sciences University of Hokkaido, Ishikari-Tobetsu, N/A, Japan
)
SESSION INFORMATION
Poster
Periodontal disease/ Molecular Aspects of Fibroblasts
03/14/2003