The development of models using techniques capable of minimizing artifacts, reducing total work time, and producing highly reliable data are of continuing interest to our research efforts. Objective: The purpose of this study was to determine the applicability of an optical fluorescence technique (QLF, Inspektor Research Systems) for conducting
in vitro model studies in a more time efficient manner. Methods: Cores of human enamel were prepared and mounted using standard procedures. Baseline images using the QLF system were taken on each specimen. Specimens were then exposed to demineralization media for a period of a)24, b)48, or c)72 hours. QLF images were again obtained following demineralization. Specimens were then placed in pooled human saliva and lesions followed in time to determine if reversal occurred. QLF images were captured at 2, 24, 48, and 72 hours. Results:
DQ values for each time point were: a) -27.6, -10.2, -6.1, -4.8; b) -55.4, -37.2, -7.9, -7.3; c)–64.8, -58.1, -36.8, -26.3. By the 72 hour remineralization time point,
DQ demonstrated a=b>c. Future use of this model to detect product differences is advised using only the 72 hour lesion. The lesions remineralized so quickly with the 24 and 48-hour lesions that product differences would not likely be detected. Conclusions: The QLF system was able to track remineralization of three severities of lesions. As
in vitro QLF and TMR results have been demonstrated to correlate well, these results suggest a potential for significant reduction in time and effort with the use of QLF over conventional microradiographic based efforts. The applicability of optical fluorescence as a tool for tracking changes in early caries lesions has been demonstrated.