Curcumin and Bisdimethyl Curcumin Isolated from Curcuma longa Inhibit the Growth of Antibiotic-resistant Bacteria
Objective: Antibiotic resistance is the one of important problem in dental and medical fields. Therefore, new agents are needed to treat methicillin-resistant Staphylococcus aureus (MRSA) from acute oral infection.. In the present study, we investigated the antimicrobial activity of Curcuma longa (C. longa) and the antibacterial components, curcumin and bisdemethyl curcumin, from C. longa has been isolated and identified by MS, 1H-NMR, and 13C-NMR. Methods: C. longa was extracted with 80% MeOH, the extract was concentrated in vacuo, and the residue partitioned between H2O and CHCl3, EtOAc, and n-BuOH, respectively. The fractions of CHCl3 were assayed for growth inhibitory activity against MRSA. Column chromatography of the most active fraction on silica gel, Sephadex LH20, and prep-HPLC gave purified compounds. The structure of compounds was identified by MS, 1H-NMR, and 13C-NMR. Results: The CHCl3 fraction from solvent partition possessed greater antibacterial potential than other fractions. The fractions were obtained from column chromatography with silica gel and Sephadex LH20 had antibacterial effect, and the fraction was separated by prep-HPLC to purified compounds. The compounds were identified as curcumin and bisdemethyl curcumin by MS, 1H-NMR, and 13C-NMR. They had antibacterial activity against the MRSA. Conclusion: These results suggest that curcumin and bisdemethyl isolated from C. longa by bioactivity-guided fractionation may have antibacterial activity against MRSA. This paper was supported by a grant No. R01-2001-00133 from Korea Science & Engineering Foundation and research fund.
Division: IADR/PER General Session
Meeting:2003 IADR/PER General Session (Goteborg, Sweden) Location: Goteborg, Sweden
Year: 2003 Final Presentation ID:546 Abstract Category|Abstract Category(s):Microbiology / Immunology and Infection Control
Authors
Kim, Y.c
( Wonkwang Univ, Iksan City, N/A, South Korea
)
You, Yong-ouk
( School of Dentistry, Wonkwang University, Iksan, N/A, South Korea
)
Jeong, S.l.
( School of Dentistry, Wonkwang University, Iksan, N/A, South Korea
)
Yu, H.h.
( School of Dentistry, Wonkwang University, Iksan, N/A, South Korea
)
Han, S.h.
( School of Dentistry, Wonkwang University, Iksan, N/A, South Korea
)
Kim, Kang-ju
( School of Dentistry, Wonkwang University, Iksan, N/A, South Korea
)