The Antibacterial Effect of Active Point with Chlorhexidine and Calcium Hydroxide Point

Objectives: The objective of the study was to evaluate the antibacterial effect of activ point with chlorhexidine (ACP, Roeko, Langenau, Germany) and calcium hydroxide point (CHP, Roeko, Langenau, Germany) in mixed cultures of E. faccalis, S. aureus and S mitis. Methods: 10⁷ CFU/ml of each strain were suspended in 50% horse serum. 200 ml of aliquots of this mixed culture were transferred into an separated Eppendorf tube containing ACP or CHP. The antibacterial effect was assessed after incubation in 39 degree centrigrade for 1,7 and 14 days respectively. 20 ml of aliquots were taken from each Eppendorf tube for determination of remained bacterial colonies. CFU/ml of each bacterial strain was counted by using colony counter on selective agar medium that was used to differentiate each strain of bacterium. Results: The results showed that remained bacteria in ACP and CHP were less than the control group with no point after 7 days observation(p<0.05). The number of three bacterial strains were reduced in ACP more than CHP in 7 and 14 days observation. Moreover no bacteria were found after contacting with ACP over 7 days. Conclusion: In conclusion ACP seems suitable as an in vitro intracanal medication . However further reseach should be studied in vivo to investigate its clinical efficiency.