Methods: Six dentin bonding agents (Mac Bond, One-Up Bond F, Clearfil SE Bond, FL Bond, Prompt L-Pop and Prime&Bond NT ) were used. The efficacy of bonding agents were compared to that of epinephrine (control) in the viewpoint of the concentration applied and the contraction forces induced on the smooth muscle were recorded using a force displacement transducer. Dose dependent relaxations caused by the test materials were recorded in the same manner in comparison with papaverin. Differences in either contraction or relaxation forces between the bonding agent groups were determined by Friedman and Wilcoxon Signed ranks tests ( p=0.01 ).
Results: FL Bond, SE Bond, One Up Bond, Mac Bond produced dose dependent relaxations. Differences between these materials and papaverin for concentration-dependent relaxations were not statistically significant ( p>0.01 ) Prompt L-Pop and Prime&Bond NT produced epinephrine-like contractions on the rat carotid artery ( smooth muscle ) and the differences between the contraction force of these two adhesives were not significant ( p>0.01) As expected, epinephrine produced concentration-dependent contractions on the carotid artery for each group (P< 0.01).There was also a statistically-significant relationship between each bonding agent and epinephrine for concentration-dependent contractions ( P<0.01 )
Conclusions: For self-etching primers which produce vascular relaxation, hemorrhage control with additional chemicals may be considered if direct pulp capping is to be performed using such adhesive systems.