IADR Abstract Archives
Is the 5% Iodine Povidone Treatment Effective in the Staphylococcus aureus Nasal Decolonization?
Objectives: Evaluated the efficiency of 5% Iodine povidone treatment in the Staphylococcus aureus nasal decolonization in a dental student population.
Methods: The UNIBE ethics department approved this study, were 65 dental students from Universidad Iberoamericana (Dominican Republic) were recluted. A swab was inserted into each nostril approximately 1.5 cm deep and rotated 5 times each time, using a sterile Transystem swab with the Amies Agar Gel transport.
All the samples were cultivated by the striate technique with the mannitol salt agar at 37 ° C for 24-48 hours.
After the bacterial growth, the culture media with SA showed a yellowish color due to the fermentation of the mannitol, indicating a positive result. A Gram stain was performed to observe Gram-positive cocci arranged in clusters.
Them a Catalase was performed to confirm the genus Staphylococcus, and coagulase to confirm the species aureus, resulting in 22 positive students, 3 were discarded, and 1 decided to withdraw from the study. The final sample was 18 students.
Them the Kirby-Bauer standardized method was used to evaluate the susceptibility to oxacillin and vancomycin.
It was mixed 100 ml of 10% Iodine povidone with 100 ml of sterile water to achieve a concentration of 5% Iodine povidone. A loaded atomizer was placed at the entrance of each nostril. This procedure was repeated for 5 days with a daily application. Then another sample was collected, Gram stain, biochemical tests and antibiogram were repeated to assess whether the treatment was effective.
Results: From the 18 students who performed the treatment with 5% Iodine povidone, 5 were decolonized and 13 continued colonized 61% of these were male.
Conclusions: Treatment with 5% Iodine povidone was not effective since 72% participants continue to be colonized after completing the treatment cycle.
Methods: The UNIBE ethics department approved this study, were 65 dental students from Universidad Iberoamericana (Dominican Republic) were recluted. A swab was inserted into each nostril approximately 1.5 cm deep and rotated 5 times each time, using a sterile Transystem swab with the Amies Agar Gel transport.
All the samples were cultivated by the striate technique with the mannitol salt agar at 37 ° C for 24-48 hours.
After the bacterial growth, the culture media with SA showed a yellowish color due to the fermentation of the mannitol, indicating a positive result. A Gram stain was performed to observe Gram-positive cocci arranged in clusters.
Them a Catalase was performed to confirm the genus Staphylococcus, and coagulase to confirm the species aureus, resulting in 22 positive students, 3 were discarded, and 1 decided to withdraw from the study. The final sample was 18 students.
Them the Kirby-Bauer standardized method was used to evaluate the susceptibility to oxacillin and vancomycin.
It was mixed 100 ml of 10% Iodine povidone with 100 ml of sterile water to achieve a concentration of 5% Iodine povidone. A loaded atomizer was placed at the entrance of each nostril. This procedure was repeated for 5 days with a daily application. Then another sample was collected, Gram stain, biochemical tests and antibiogram were repeated to assess whether the treatment was effective.
Results: From the 18 students who performed the treatment with 5% Iodine povidone, 5 were decolonized and 13 continued colonized 61% of these were male.
Conclusions: Treatment with 5% Iodine povidone was not effective since 72% participants continue to be colonized after completing the treatment cycle.