IADR Abstract Archives
Comparative MicroCT Assessment of Enamel-Demineralization Prevention of Silver-Diamine-Fluoride and Fluoride-Varnish
Objectives: The ability of SDF to enhance the resistance of sound enamel to demineralization has not been studied by micro-CT. Micro-CT provides a nondestructive method to assess the ion-retention pattern (mineral density) pre and post treatment. This study aims to compare the preventive effect of SDF to fluoride varnish using Micro-CT.
Methods: Eighteen human-teeth were sectioned mesio-distally and randomly assigned to control (artificial saliva-no treatment), FV, and SDF treatment groups (n=12/group). 2 x 3 mm treatment window was made using acid-resistant nail polish. Two positioning holes were made by a diamond bur adjacent to each window to help determine the exact location during Micro-CTscanning at baseline and post-pH cycling. Baseline mineral-density was recorded using MicroCT at 8.3 micrometer resolution. Following treatment, specimens underwent 9-day pH-cycle (8 days demineralization and remineralization solution + 1-day). On day 4, solutions in the tubes were replaced with fresh solutions. On day 9, specimens were kept in the remineralization solution for 24 hours. A second Micro-CT scanning to record post-treatment mineral density was carried out.
Results: Paired T-test was used to compare the mean change in mineral-density within each group. The baseline density was similar in all groups. Following pH-cycle all groups demonstrated significant increase in mineral-density (p<0.05) comparing baseline to post-cycle values. ANOVA followed by Student-Newman-Keulswere used to compare the mean differences of all groups. SDF showed a significant increase in the mean mineral-density compared to other groups (p=0.005).
Conclusions: Within the limitations of this study, results suggest that SDF significantly enhances resistance of sound enamel to demineralization and significantly outperformed FV treatment.
Supported by Texas A&M Health Sciences and Baylor Oral Health Foundation (MTM).
Methods: Eighteen human-teeth were sectioned mesio-distally and randomly assigned to control (artificial saliva-no treatment), FV, and SDF treatment groups (n=12/group). 2 x 3 mm treatment window was made using acid-resistant nail polish. Two positioning holes were made by a diamond bur adjacent to each window to help determine the exact location during Micro-CTscanning at baseline and post-pH cycling. Baseline mineral-density was recorded using MicroCT at 8.3 micrometer resolution. Following treatment, specimens underwent 9-day pH-cycle (8 days demineralization and remineralization solution + 1-day). On day 4, solutions in the tubes were replaced with fresh solutions. On day 9, specimens were kept in the remineralization solution for 24 hours. A second Micro-CT scanning to record post-treatment mineral density was carried out.
Results: Paired T-test was used to compare the mean change in mineral-density within each group. The baseline density was similar in all groups. Following pH-cycle all groups demonstrated significant increase in mineral-density (p<0.05) comparing baseline to post-cycle values. ANOVA followed by Student-Newman-Keulswere used to compare the mean differences of all groups. SDF showed a significant increase in the mean mineral-density compared to other groups (p=0.005).
Conclusions: Within the limitations of this study, results suggest that SDF significantly enhances resistance of sound enamel to demineralization and significantly outperformed FV treatment.
Supported by Texas A&M Health Sciences and Baylor Oral Health Foundation (MTM).
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