IADR Abstract Archives
Indirect Pulp Capping: Effect on Pulp Cell Survival and Proliferation
Objectives: This study compared the effects of GLUMA and Theracal-LC applied within 0.5mm from the pulp on pulp cell viability.
Methods: Non-carious third molars were obtained and stored in .5% Chloramine-T for up to 3 months at 4° C. Teeth had a 2.1mm X 1.5mm wide cylinder cut from the coronal dentin, using a sterile diamond bur. The cylinder had a central hole drilled to within 0.5 mm of the roof of the pulp chamber – see figure.
Cylinders were soaked in 100% NaOCl for 5 minutes, removing any organic material, and were rinsed for multiple washes in sterile RO water in a BSL-2 hood over 1 month. Treatments shown in Table 1 were applied to the interior surface of the tooth cylinders and they were placed in Transwell® cell culture inserts within 24-well culture plates (n=9 each treatment). The volume of fluid prevented media from entering the tooth cylinders but allowed ions and other soluble products to diffuse into the culture well. Mouse dental pulp cells (MDPC-23) were seeded at 10,000 cells/well and incubated below the Transwell® inserts. DMEM was changed daily for 14 days, and cell metabolic rate and viability were measured every 48 hours using 10% Alamar Blue in media. An ANOVA with post-hoc Tukey test were used to test for significance (α=0.05).
Results: Wells treated with Gluma in the media were significantly lower than all other treatments at all times.
Wells treated with Gluma and Theracal in the tooth had lower cell viability compared to wells where the teeth had Theracal only. Wells with Theracal in the media had higher cell counts than wells with Theracal and Gluma in the tooth.
Conclusions: Clinically, 0.5 mm of dentin, after decay removal, will not prevent diffusion of the beneficial effects of TheraCal. However, addition of Gluma does prevent the ion diffusion from occurring.
Methods: Non-carious third molars were obtained and stored in .5% Chloramine-T for up to 3 months at 4° C. Teeth had a 2.1mm X 1.5mm wide cylinder cut from the coronal dentin, using a sterile diamond bur. The cylinder had a central hole drilled to within 0.5 mm of the roof of the pulp chamber – see figure.
Cylinders were soaked in 100% NaOCl for 5 minutes, removing any organic material, and were rinsed for multiple washes in sterile RO water in a BSL-2 hood over 1 month. Treatments shown in Table 1 were applied to the interior surface of the tooth cylinders and they were placed in Transwell® cell culture inserts within 24-well culture plates (n=9 each treatment). The volume of fluid prevented media from entering the tooth cylinders but allowed ions and other soluble products to diffuse into the culture well. Mouse dental pulp cells (MDPC-23) were seeded at 10,000 cells/well and incubated below the Transwell® inserts. DMEM was changed daily for 14 days, and cell metabolic rate and viability were measured every 48 hours using 10% Alamar Blue in media. An ANOVA with post-hoc Tukey test were used to test for significance (α=0.05).
Results: Wells treated with Gluma in the media were significantly lower than all other treatments at all times.
Wells treated with Gluma and Theracal in the tooth had lower cell viability compared to wells where the teeth had Theracal only. Wells with Theracal in the media had higher cell counts than wells with Theracal and Gluma in the tooth.
Conclusions: Clinically, 0.5 mm of dentin, after decay removal, will not prevent diffusion of the beneficial effects of TheraCal. However, addition of Gluma does prevent the ion diffusion from occurring.
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